Activity-based PIKFYVE lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.
Target Overview
CAT#
PIKFYVE
Enzyme
Human PIP5K3
HGNC
PIKFYVE
Synonym
KIAA0981, PIP5K3
Scientific Information
Q9Y2I7 (www.uniprot.org/uniprotkb/Q9Y2I7)
Assay Properties
Substrates
PI(3)P:PS, 25 µM
ATP, 10 µM
ATP, 10 µM
Reaction
1-phosphatidyl-1D-myo-inositol 3-phosphate + ATP = 1-phosphatidyl-1D-myo-inositol 3,5-biphosphate + ADP
Detection
ADP-Glo™ Assay
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| YM-201636 | 23 |
| APY0201 | 1 |
| AG-183 | 3,800 |
Further Information
More information can be found on our website Kinase Assays
Screening Location
Malvern, PA, USA
Reference compound IC50 for PIKFYVE
PDF