PIM2 Kinase Assay Service

Activity-based PIM2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
PIM2

Enzyme
Human PIM2

HGNC
PIM2

Synonym
PIM2

Substrates
Peptide substrate, [RSRHSSYPAGT], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 29
Ro 31-8220 750
GW 5074 50
Rottlerin 6,500
Scientific Information
Q9P1W9 (http://www.uniprot.org/uniprot/Q9P1W9)

Screening Location
Malvern, USA

Reference compound IC50 for PIM2
Reference compound IC50 for PIM2