Activity-based PIM3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PIM3
Enzyme
Human PIM3
HGNC
PIM3
Synonym
PIM3
Substrates
Peptide substrate, [RSRHSSYPAGT], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | <0.1 |
| Ro 31-8220 | 12.9 |
| GW 5074 | 40 |
| H-89 | 31,200 |
| Rottlerin | 2,500 |
Scientific Information
Q86V86 (http://www.uniprot.org/uniprot/Q86V86)
Screening Location
Malvern, USA
Reference compound IC50 for PIM3
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