Activity-based PIP4K2A lipid kinase assay for compound screening and profiling via ADP-GloTM kinase assay.
Target Overview
CAT#
PIP4K2A
Enzyme
Human PIP4K2A
HGNC
PIP4K2A
Synonym
PI42A, PI5P4KA, PIP5K2, PIP5K2A, PIP5KIII
Scientific Information
P48426 (https://www.uniprot.org/uniprotkb/P48426)
Assay Properties
Substrates
PI(5)P:PS, 50 µM
ATP, 10 µM
Reaction
1-phosphatidyl-1D-myo-inositol 5-phosphate + ATP = 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| BAY-091 | 2.8 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for PIP4K2A
