Activity-based PIP4K2C lipid kinase assay for compound screening and profiling via ADP-GloTM kinase assay.
Target Overview
CAT#
PIP4K2C
Enzyme
Human PIP4K2C
HGNC
PIP4K2C
Synonym
PI42C, PIP5K2C
Scientific Information
Q8TBX8 (https://www.uniprot.org/uniprotkb/Q8TBX8)
Assay Properties
Substrates
PI(5)P:PS, 50 µM
ATP, 10 µM
Reaction
1-phosphatidyl-1D-myo-inositol 5-phosphate + ATP = 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| BAY-091 | 0.7 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for PIP4K2C
