PIP5K1A Kinase Assay Service

Activity-based PIP5K1A lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.
Target Overview

CAT#
PIP5K1A

Enzyme
Human PIP5K1A

HGNC
PIP5K1A

Synonym
PIP5K1-alpha, PIP5KIalpha, PtdIns(4)P-5-kinase 1 alpha

Substrates
PI(4)P:PS, 50 µM
ATP 10 µM

Assay Properties

Reaction
1-phosphatidyl-1D-myo-inositol 4-phosphate + ATP = 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
PIK-93 88,000
Scientific Information
Q99755 (http://www.uniprot.org/uniprot/Q99755)

Screening Location
Malvern, USA

Reference compound IC50 for PIP5K1A
Reference compound IC50 for PIP5K1A