Activity-based PIP5K1B lipid kinase assay for compound screening and profiling via ADP-GloTM kinase assay.
Target Overview
CAT#
PIP5K1B
Enzyme
Human PIP5K1B
HGNC
PIP5K1B
Synonyms
STM7, PIP5K1-beta
Scientific Information
O14986 (www.uniprot.org/uniprotkb/O14986)
Assay Properties
Substrate
PI(4)P, 10 µM ATP, 10 µM
Reaction
1-phosphatidyl-1D-myo-inositol 4-phosphate + ATP = 1-phosphatidyl-1D-myo-inositol 4,5-biphosphate + ADP
Detection
ADP-GloTM Assay
Reference Compound IC50s
| Compound | IC50 (µM) |
|---|---|
| APY0201 | 2 |
| PIK-93 | 38 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for PIP5K1B
