Activity-based PKA kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PKA
Enzyme
Human PKA
HGNC
PRKACA
Synonym
PKACa
Substrates
Peptide substrate, [LCGRTGRRNSI], 100 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
1-phosphatidyl-1D-myo-inositol 4-phosphate + ATP = 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1.5 |
| Ro 31-8220 | 675 |
| GW5074 | >100,000 |
| H-89 | 48 |
| Rottlerin | >100,000 |
Scientific Information
P17612 (http://www.uniprot.org/uniprot/P17612)
Screening Location
Malvern, USA
Reference compound IC50 for PKA
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