Activity-based PKA (L206R) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PKA (L206R)
Enzyme
Human PKA (L206R)
HGNC
PRKACA (L206R)
Synonym
cAMP-dependent protein kinase catalytic subunit alpha, PRKACA, PKACA, KAPCA, PKACa
Substrates
Peptide substrate, [LCGRTGRRNSI], 100 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.6 |
| Ro 31-8220 | 94 |
| GW5074 | 10,400 |
| H-89 | 21 |
| Rottlerin | >100,000 |
Scientific Information
P17612 (http://www.uniprot.org/uniprot/P17612)
Screening Location
Malvern, USA
Reference compound IC50 for PKA (L206R)
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