PKAcb Kinase Assay Service

Activity-based PKAcb kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PKAcb
Enzyme
Human PKAcb
HGNC
PRKACB
Synonym
PKAcb, MGC41879, MGC9320, PKA c-beta
Substrates
Peptide substrate, [KEAKEKRQEQIAKRRRLSSLRASTSKSGGSQK], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine2.1
Ro 31-8220250
H-89220
Scientific Information
P22694 (http://www.uniprot.org/uniprot/P22694)
Screening Location
Malvern, USA
Reference compound IC50 for PKAcb
Reference compound IC50 for PKAcb