PKAcg Kinase Assay Service

Activity-based PKAcg kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
PKAcg

Enzyme
Human PKAcg

HGNC
PRKACG

Synonym
PKAcg

Substrates
Peptide substrate, [KEAKEKRQEQIAKRRRLSSLRASTSKSGGSQK], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 9.3
Ro 31-8220 3,700
H-89 630
Scientific Information
P22612 (http://www.uniprot.org/uniprot/P22612)

Screening Location
Malvern, USA

Reference compound IC50 for PKAcg
Reference compound IC50 for PKAcg