PKCalpha Kinase Activity Assay Service

Activity-based PKCalpha kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview

RBE Target ID

PKCalpha

Enzyme

Human PKCalpha, wt

HGNC

PRKCA

Manning ID

PKCa

Synonym

PKC-alpha, PKCA, PRKACA, PRKCA, PKc-alpha, PKCalpha, PKCa

UniProt

P17252 (KPCA_HUMAN)

Kinase Type

Protein kinase

Kinase Group

AGC

Assay Properties

Assay Format

33PanQinaseTM

Substrates

3xChocktide-PKC(19-31), ATP

Reaction

3xChocktide-PKC(19-31) + 33P-ATP = 33P-3xChocktide-PKC(19-31) + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 3
Sorafenib 10000
Sunitinb 37000

Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability

Screening Opportunities
FreeChoice PKCalpha
345 wt Profiler Yes
345 IC50 wt Profiler Yes
Mutant Profiler Addon No
Lipid Kinase Profiler Addon No
IC50 Profiler No
Recombinant Protein Availability
Active recombinant protein

Yes; ProQinaseTM – PKCalpha
(Activity verified by 33PanQinaseTM-Assay)

Product Number

0222-0000-1

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