Activity-based PKCeta kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PKCeta
Enzyme
Human PKCeta
HGNC
PRKCH
Synonym
PKC-L, PKCL, PRKCL, nPKC-eta
Substrates
Peptide substrate, [ERMRPRKRQGSVRRRV], 20 µM, Lipid activator,
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.4 |
| Ro 31-8220 | <1.0 |
| GW5074 | 11,000 |
| H-89 | 17.8 |
| Rottlerin | >100,000 |
Scientific Information
P24723 (http://www.uniprot.org/uniprot/P24723)
Screening Location
Malvern, USA
Reference compound IC50 for PKCeta
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