PKCg Kinase Assay Service

Activity-based PKCg kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
PKCg

Enzyme
Human PKCg

HGNC
PRKCG

Synonym
PKCC, PKCG, SCA14, MGC57564, PKC-gamma

Substrates
Peptide substrate [ERMRPRKRQGSVRRRV], 20 µM, and Lipid activatorATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.5
Ro 31-8220 <1.0
GW5074 2,200
H-89 280
Rottlerin >100,000
Scientific Information
P05129 (http://www.uniprot.org/uniprot/P05129)

Screening Location
Malvern, USA

Reference compound IC50 for PKCg
Reference compound IC50 for PKCg