Activity-based PKCiota kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PKCiota
Enzyme
Human PKCiota
HGNC
PRKCI
Synonym
PKCI, DXS1179E, MGC26534, nPKC-iota
Substrates
Peptide substrate, [ERMRPRKRQGSVRRRV], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 6.0 |
| Ro 31-8220 | 94.3 |
| H-89 | 16,700 |
Scientific Information
P41743 (http://www.uniprot.org/uniprot/P41743)
Screening Location
Malvern, USA
Reference compound IC50 for PKCiota
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