Activity-based PKCmu/PRKD1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PKCmu/PRKD1
Enzyme
Human PKCmu/PRKD1
HGNC
PRKD1
Synonym
PKD3, EPK2, PKc-NU, PKD3, PRKCN
Scientific Information
Q15139 (http://www.uniprot.org/uniprot/Q15139)
Assay Properties
Substrates
Peptide substrate, [KKLNRTLSVA], 20 µM
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 |
|---|---|
| Staurosporine | 1.6 nM |
| Ro 31-8220 | 58.2 nM |
| GW 5074 | 4.1 µM |
| H-89 | 0.8 µM |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Screening Assay Services.
Reference compound inhibition of PKCmu/PRKD1
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