PKCnu/PRKD3 Kinase Assay Service

Activity-based PKCnu/PRKD3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
PKCnu/PRKD3

Enzyme
Human PKCnu/PRKD3

HGNC
PRKD3

Synonym
PKD3, EPK2, PKc-NU, PKD3, PRKCN

Substrates
Peptide substrate, [KKLNRTLSVA], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.6
Ro 31-8220 84
GW 5074 4,000
H-89 1,300
Scientific Information
O94806 (http://www.uniprot.org/uniprot/O94806)

Screening Location
Malvern, USA

Reference compound IC50 for PKCnu/PRKD3
Reference compound IC50 for PKCnu/PRKD3