PKCzeta Kinase Assay Service

Activity-based PKCzeta kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
PKCzeta

Enzyme
Human PKCzeta

HGNC
PRKCZ

Synonym
PKC2

Substrates
Peptide substrate, [ERMRPRKRQGSVRRRV], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 87
Ro 31-8220 138
H-89 3,500
Scientific Information
Q05513 (http://www.uniprot.org/uniprot/Q05513)

Screening Location
Malvern, USA

Reference compound IC50 for PKCzeta
Reference compound IC50 for PKCzeta