PKD2/PRKD2 (G848E) Kinase Assay Service

Activity-based PKD2/PRKD2 (G848E) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview

CAT#

PKD2/PRKD2 (G848E)

Enzyme

Human PKD2/PRKD2 (G848E)

HGNC

PKD2/PRKD2 (G848E)

Synonym

Serine/threonine-protein kinase D2, nPKC-D2, KPCD2, PRKD2

Scientific Information

Q9BZL6 (http://www.uniprot.org/uniprot/Q9BZL6)

Assay Properties

Substrates

Peptide substrate, [KKLNRTLSVA], 20 µM
ATP, 10 µM

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.8
Ro 31-8220 210
GW5074 2,900
H-89 1,100
Rottlerin 20,000

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 for PKD2/PRKD2 (G848E)

Reference compound IC50 for PKD2/PRKD2 (G848E)