Activity-based PKD2 (G870E) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PKD2 (G870E)
Enzyme
Human PKD2 (G870E)
HGNC
PRKD2 (G870E)
Synonym
PKD2, HSPC187
Substrates
Peptide substrate, [KKLNRTLSVA], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1.0 |
| Ro 31-8220 | 350 |
| GW5074 | 11,000 |
| H-89 | 1,600 |
| Rottlerin | 7,500 |
Scientific Information
Q9BZL6 (http://www.uniprot.org/uniprot/Q9BZL6)
Screening Location
Malvern, USA
Reference compound IC50 for PKD2 (G870E)
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