PKD2/PRKD2 Kinase Assay Service

Activity-based PKD2/PRKD2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
PKD2

Enzyme
Human PKD2

HGNC
PRKD2

Synonym
PKD2, HSPC187

Substrates
Peptide substrate, [KKLNRTLSVA], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.47
Ro 31-8220 263
GW 5074 4,000
H-89 2,300
Scientific Information
Q9BZL6 (http://www.uniprot.org/uniprot/Q9BZL6)

Screening Location
Malvern, USA

Reference compound IC50 for PKD2/PRKD2
Reference compound IC50 for PKD2/PRKD2