PKG1a Kinase Assay Service

Activity-based PKG1a kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PKG1α
Enzyme
Human PKG1α
HGNC
PRKG1
Synonym
PKG1, PGK, PRKG1B, PRKGR1B
Substrates
Peptide substrate, [LRRASLG], 100 µM
ATP 1 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine0.86
Ro 31-822029
GW 507410,000
H-8955
Scientific Information
Q13976 (http://www.uniprot.org/uniprot/Q13976)
Screening Location
Malvern, USA
Reference compound IC50 for PKG1α
Reference compound IC50 for PKG1α