Activity-based PKG2/PRKG2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PKG2
Enzyme
Human PKG2
HGNC
PRKG2
Synonym
PKG2, cGKII, PRKGR2
Substrates
Peptide substrate, [LRRASLG], 100 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.4 |
| Ro 31-8220 | 5.6 |
| H-89 | 390 |
Scientific Information
Q13237 (http://www.uniprot.org/uniprot/Q13237)
Screening Location
Malvern, USA
Reference compound IC50 for PKG2/PRKG2
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