Activity-based PKMYT1 kinase assay for compound screening and profiling via Probe-Displacement kinase assay.
Target Overview
CAT#
PKMYT1 (Binding)
Enzyme
Human PKMYT1
HGNC
PKMYT1
Synonym
Myt1 kinase, MYT1, PMYT1
Assay Properties
Probe
Fluorescent Kinase Tracer based on ATP-competitive kinase inhibitors, 25 nM
Assay Format
Binding interaction between fluorescently Kinase Tracer and Kinase is quantified by HTRF technology using anti Tag HTRF reagents. Compounds binding in this binding pocket displace fluorescently labeled probe resulting loss of binding signal.
Readout
HTRF Readout, Probe-Displacement
Reference Compound IC50s
| Compounds | IC50 |
|---|---|
| Lunresertib (RP-6306) | 1.2 nM |
| CTX-0294885 | 6.6 µM |
| Staurosporine | >10 µM |
Scientific Information
Q99640 (http://www.uniprot.org/uniprot/Q99640)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound inhibition of PKMYT1
