PKN1-ANXA4 (Aex2Pex13) Kinase Assay Service

Activity-based PKN1-ANXA4 (Aex2Pex13) kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

PKN1, ANXA4 (Aex2Pex13)

Enzyme

Human PKN1-ANXA4 (Aex2Pex13)

HGNC

PKN1-ANXA4 (Aex2Pex13)

Synonym

ANXA4: Annexin A4, 35-beta calcimedin, Carbohydrate-binding protein p33/p41, Chromobindin-4
PKN1: Serine/threonine-protein kinase N1, Protein kinase C-like PKN,Protein kinase PKN-alpha, PKN, PRK1, PRKCL1

Scientific Information

PKN1: Q16512 (http://www.uniprot.org/uniprot/Q16512)
ANXA4: P09525 (http://www.uniprot.org/uniprot/P09525)

Assay Properties

Substrates

Peptide substrate, [KKLNRTLSVA], 20 µM
ATP, 10 µM

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Compounds	IC50 (nM)
Staurosporine	<0.1
Ro 31-8220	3.2
GW5074	3,600
H-89	40
Rottlerin	>100,000

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 for PKN1-ANXA4 (Aex2Pex13)