PKN1-TECR (Tex1Pex10) Kinase Assay Service

Activity-based PKN1-TECR (Tex1Pex10) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
PKN1, TECR (Tex1Pex10)-

Enzyme
Human TECR-PKN1 (Tex1Pex10)

HGNC
PKN1-TECR

Synonym
DBK, PKN, PAK1, PRK1, PRKCL1, MGC46204, TECR-PKN1

Substrates
Peptide substrate, [KKLNRTLSVA], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine <0.1
Ro 31-8220 2.1
GW 5074 >100,000
H-89 25
Rottlerin >100,000
Scientific Information
TECR: Q9NZ01 (http://www.uniprot.org/uniprot/Q9NZ01)PKN1: Q16512 (http://www.uniprot.org/uniprot/Q16512)

Screening Location
Malvern, USA

Reference compound IC50 for PKN1-TECR (Tex1Pex10)
Reference compound IC50 for PKN1-TECR (Tex1Pex10)

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