PKN1/PRK1 Kinase Assay Service

Activity-based PKN1/PRK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
PKN1

Enzyme
Human PKN1

HGNC
PKN1

Synonym
DBK, PKN, PAK1, PRK1, PRKCL1, MGC46204

Substrates
Peptide substrate, [KKLNRTLSVA], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine <0.1
Ro 31-8220 2.3
GW 5074 5,600
H-89 79
Scientific Information
Q16512 (http://www.uniprot.org/uniprot/Q16512)

Screening Location
Malvern, USA

Reference compound IC50 for PKN1/PRK1
Reference compound IC50 for PKN1/PRK1