Activity-based PLK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PLK1
Enzyme
Human PLK1
HGNC
PLK1
Synonym
STPK13, PLK
Scientific Information
P53350 (http://www.uniprot.org/uniprot/P53350)
Assay Properties
Substrates
Casein, 1 mg/ml
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 |
|---|---|
| Staurosporine | 156 nM |
| GW 5074 | 2.7 µM |
| Rottlerin | 22.7 µM |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound inhibition of PLK1
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