Activity-based PLK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PLK1
Enzyme
Human PLK1
HGNC
PLK1
Synonym
STPK13
Substrates
Casein, 1 mg/ml
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 350 |
| Ro 31-8220 | 180,000 |
| GW 5074 | 26,000 |
| Rottlerin | 26,600 |
Scientific Information
P53350 (http://www.uniprot.org/uniprot/P53350)
Screening Location
Malvern, USA
Reference compound IC50 for PLK1
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