Activity-based PLK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PLK3
Enzyme
Human PLK3
HGNC
PLK3
Synonym
CNK, FNK, PRK
Substrates
Casein, 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | >100,000 |
| Ro 31-8220 | >100,000 |
| GW 5074 | >100,000 |
| H-89 | >100,000 |
| BI2536 | 5.5 |
Scientific Information
Q9H4B4 (http://www.uniprot.org/uniprot/Q9H4B4)
Screening Location
Malvern, USA
Reference compound IC50 for PLK3
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