PLK4/SAK Kinase Assay Service

Activity-based PLK4/SAK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
PLK4/SAK

Enzyme
Human PLK4/SAK

HGNC
PLK4

Synonym
SAK, STK18

Substrates
Casein, 2 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 4.2
Ro 31-8220 15,000
GW5074 4,700
H-89 >50,000
Rottlerin >50,000
Scientific Information
O00444 (http://www.uniprot.org/uniprot/O00444)

Screening Location
Malvern, USA

Reference compound IC50 for PLK4/SAK
Reference compound IC50 for PLK4/SAK