PLK4/SAK Kinase Assay Service

Activity-based PLK4/SAK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PLK4/SAK
Enzyme
Human PLK4/SAK
HGNC
PLK4
Synonym
SAK, STK18
Substrates
Casein, 2 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine4.2
Ro 31-822015,000
GW50744,700
H-89>50,000
Rottlerin>50,000
Scientific Information
O00444 (http://www.uniprot.org/uniprot/O00444)
Screening Location
Malvern, USA
Reference compound IC50 for PLK4/SAK
Reference compound IC50 for PLK4/SAK