PP1A Phosphatase Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
PP1A
Enzyme
Human PP1A
Synonyms
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PPP1CA, PPP1A
Substrates
Fluorogenic substrate DiFMUP, 10 μM
Assay Properties
Reaction
[a protein]-serine/threonine phosphate + H₂O = [a protein]-serine/threonine + phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| NSC-87877 | >100,000 |
| PHPS1 | >100,000 |
| PTP1B Inhibitor | 24,000 |
| Cantharidic Acid | 560 |
Scientific Information
P62136 (http://www.uniprot.org/uniprot/P62136)
Screening Location
Malvern, USA
Reference Compound IC50 for PP1A
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