PP1B Phosphatase Assay Service

PP1B Phosphatase Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
PP1B
Enzyme
Human PP1B
Synonyms
Serine/threonine-protein phosphatase PP1-beta catalytic subunit, PPP1CB)
Substrates
Fluorogenic substrate DiFMUP, 30 μM
Assay Properties
Reaction
[a protein]-serine/threonine phosphate + H₂O = [a protein]-serine/threonine + phosphate
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
NSC-8787781,000
PHPS1>100,000
PTP1B Inhibitor23,000
Cantharidic Acid570
Scientific Information
P62140 (http://www.uniprot.org/uniprot/P62140)
Screening Location
Malvern, USA
Reference Compound IC50 for PP1B
Reference Compound IC50 for PP1B