PP2A alpha/PPP2R1A Complex Phosphatase Assay Service

PP2A alpha/PPP2R1A Complex Phosphatase Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview

CAT#
PP2A alpha/PPP2R1A Complex

Enzyme
Human PP2Aalpha/PPP2R1A Complex

Synonyms
PP2A alpha: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PPP2CA, PPP2R1A: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform

Substrates
Fluorogenic substrate DiFMUP, 10 μM

Assay Properties

Reaction
[a protein]-serine/threonine phosphate + H₂O = [a protein]-serine/threonine + phosphate

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
PHPS1 1,100
PTP1B Inhibitor 17,700
Cantharidic acid 70
Scientific Information
PP2A alpha: P67775 (http://www.uniprot.org/uniprot/P67775)PPP2R1A: P30153 (http://www.uniprot.org/uniprot/P30153)

Screening Location
Malvern, USA

Reference Compound IC50 for PP2A alpha/PPP2R1A Complex
Reference Compound IC50 for PP2A alpha/PPP2R1A Complex

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