PP2A alpha/PPP2R1A Complex Phosphatase Assay Service

PP2A alpha/PPP2R1A Complex Phosphatase Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
PP2A alpha/PPP2R1A Complex
Enzyme
Human PP2Aalpha/PPP2R1A Complex
Synonyms
PP2A alpha: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PPP2CA, PPP2R1A: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
Substrates
Fluorogenic substrate DiFMUP, 10 μM
Assay Properties
Reaction
[a protein]-serine/threonine phosphate + H₂O = [a protein]-serine/threonine + phosphate
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
PHPS11,100
PTP1B Inhibitor17,700
Cantharidic acid70
Scientific Information
PP2A alpha: P67775 (http://www.uniprot.org/uniprot/P67775)PPP2R1A: P30153 (http://www.uniprot.org/uniprot/P30153)
Screening Location
Malvern, USA
Reference Compound IC50 for PP2A alpha/PPP2R1A Complex
Reference Compound IC50 for PP2A alpha/PPP2R1A Complex