PRDM9 Methyltransferase Assay Service

Activity-based PRDM9 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
PRDM9
Enzyme
Human PRDM9
Synonym
Histone-lysine N-methyltransferase PRDM9, PR domain zinc finger protein 9, PR domain-containing protein 9, PFM6
Substrates
Histone H3, 5 µMSAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L- lysine = S-adenosyl-L-homocysteine + histone N⁶-[methyl-³H]-L- lysine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
SAH160,000
Sinefugin>200,000
BIX 01294>200,000
Chaetocin3,400
Scientific Information
Q9NQV7 (http://www.uniprot.org/uniprot/Q9NQV7)
Screening Location
Malvern, USA
Reference compound IC50 for PRDM9
Reference compound IC50 for PRDM9