Activity-based PRKX kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PRKX
Enzyme
Human PRKX
HGNC
PRKX
Synonym
PKX1
Substrates
Peptide substrate, [LRRASLG], 100 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1.1 |
| Ro 31-8220 | 380 |
| GW5074 | 13,000 |
| H-89 | 3.1 |
| Rottlerin | 19,700 |
Scientific Information
P51817 (http://www.uniprot.org/uniprot/P51817)
Screening Location
Malvern, USA
Reference compound IC50 for PRKX
PDF