PRKX Kinase Assay Service

Activity-based PRKX kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PRKX
Enzyme
Human PRKX
HGNC
PRKX
Synonym
PKX1
Substrates
Peptide substrate, [LRRASLG], 100 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine1.1
Ro 31-8220380
GW507413,000
H-893.1
Rottlerin19,700
Scientific Information
P51817 (http://www.uniprot.org/uniprot/P51817)
Screening Location
Malvern, USA
Reference compound IC50 for PRKX
Reference compound IC50 for PRKX