PRMT1 Methyltransferase Assay Service

Activity-based PRMT1 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.

Target Overview
CAT#

PRMT1

Enzyme

Human PRMT1

Synonym

Protein arginine N-methyltransferase 1, Interferon receptor 1-bound protein 4, HMT2, HRMT1L2, IR1B4, ANM1

Substrates HotSpot assay:

Histone H4, 5 µM; SAM, 1 µM

Substrates FlashPlate assay:

H4 (1-15)-Biotin, 40 nM; SAM, 1 µM

Assay Properties
Reaction

S-adenosyl-L-[methyl-³H]methionine + histone L-arginine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- arginine

Readout

Enzymatic activity

Reference Compound IC50s HotSpotTM assay
Compounds IC50 (nM)
SAH 97
Sinefugin 110
GSK 3368715 65
Reference Compound IC50s FlashPlate Assay
Compounds IC50 (nM)
SAH 5,500
Sinefugin 720
GSK 3368715 2.3
Scientific Information

Q99873 (http://www.uniprot.org/uniprot/Q99873)

Screening Location

Malvern, USA

Reference compound IC50 for PRMT1 - HotSpot
Reference compound IC50 for PRMT1 - FlashPlate