PRMT3 Methyltransferase Assay Service

Activity-based PRMT3 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
PRMT3
Enzyme
Human PRMT3
Synonym
Protein arginine N-methyltransferase 3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3, HRMT1L3, ANM3
Substrates
Histone H3, 5 µMSAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L-arginine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- arginine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
SAH665
Sinefugin775
Scientific Information
O60678 (http://www.uniprot.org/uniprot/O60678)
Screening Location
Malvern, USA
Reference compound IC50 for PRTMT3
Reference compound IC50 for PRTMT3