PRMT4 Methyltransferase Assay Service

Activity-based PRMT4 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
PRMT4
Enzyme
Human PRMT4
Synonym
Protein arginine N-methyltransferase 4, Histone-arginine methyltransferase CARM1, Coactivatorassociated arginine methyltransferase 1, CARM1
Substrates
Histone H3, 5 µMSAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L-arginine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- arginine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
SAH120
Sinefugin100
Scientific Information
Q86X55 (http://www.uniprot.org/uniprot/Q86X55)
Screening Location
Malvern, USA
Reference compound IC50 for PRMT4
Reference compound IC50 for PRMT4