Activity-based PRMT5/MEP50 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM and FlashPlate methyltranferase assay.
PRMT5: Protein arginine N-methyltransferase 5, Shk1 kinase-binding protein 1 homolog, Jak-binding protein 1, 72 kDa ICln-binding protein, HRMT1L5, IBP72, JBP1, SKB1, ANM5 MEP50: Methylosome protein 50, Androgen receptor cofactor p44 WD repeat-containing protein 77, p44/Mep50, WDR77, HKMT1069
Histone H2A, 5 µMSAM, 1 µM
H4 (1-15)-Biotin, 40 nMSAM, 1 µM
S-adenosyl-L-[methyl-³H]methionine + histone L-arginine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- arginine
Reference compound IC50 for PRMT5/MEP50 - HotSpot
Reference compound IC50 for PRMT5/MEP50 - FlashPlate