PRMT6 Methyltransferase Assay Service

Activity-based PRMT6 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
PRMT6
Enzyme
Human PRMT6
Synonym
Protein arginine N-methyltransferase 6, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6, Histone-arginine N-methyltransferase PRMT6, HRMT1L6, ANM6
Substrates
GST-GAR, 5 µMSAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L-arginine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- arginine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
SAH120
Sinefugin260
BIX01294>200,000
Chaetocin>200,000
Scientific Information
Q96LA8 (http://www.uniprot.org/uniprot/Q96LA8)
Screening Location
Malvern, USA
Reference compound IC50 for PRMT6
Reference compound IC50 for PRMT6