PRMT7 Methyltransferase Assay Service

Activity-based PRMT7 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
PRMT7
Enzyme
Human PRMT7
Synonym
Protein arginine N-methyltransferase 7, Histone-arginine N-methyltransferase PRMT7, KIAA1933
Substrates
GST-GAR, 5 µMSAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L-arginine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- arginine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
SAH340
Chaetocin144,000
Sinefungin550
Suramin>200,000
Scientific Information
Q9NVM4 (http://www.uniprot.org/uniprot/Q9NVM4)
Screening Location
Malvern, USA
Reference compound IC50 for PRMT7
Reference compound IC50 for PRMT7