PRMT8 Methyltransferase Assay Service

Activity-based PRMT8 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
PRMT8
Enzyme
Human PRMT8
Synonym
Protein arginine N-methyltransferase 8, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 4, HRMT1L3, HRMT1L4
Substrates
Histone H4, 5 µMSAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L-arginine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- arginine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
SAH198
Sinefugin173
BIX01294>100,000
Chaetocin>100,000
Scientific Information
Q9NR22 (http://www.uniprot.org/uniprot/Q9NR22)
Screening Location
Malvern, USA
Reference compound IC50 for PRMT8
Reference compound IC50 for PRMT8