PRMT8 Methyltransferase Assay Service

Activity-based PRMT8 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.

Target Overview

CAT#

PRMT8

Enzyme

Human PRMT8

Synonym

Protein arginine N-methyltransferase 8, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 4, HRMT1L3, HRMT1L4

Substrates

Histone H4, 5 µM
SAM, 1 µM

Assay Properties

Reaction

S-adenosyl-L-[methyl-³H]methionine + histone L-arginine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- arginine

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
SAH 198
Sinefugin 173
BIX01294 >100,000
Chaetocin >100,000

Scientific Information

Q9NR22 (http://www.uniprot.org/uniprot/Q9NR22)

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Methyltransferase Assays.

Reference compound IC50 for PRMT8

Reference compound IC50 for PRMT8