Activity-based PRMT8 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
PRMT8
Enzyme
Human PRMT8
Synonym
Protein arginine N-methyltransferase 8, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 4, HRMT1L3, HRMT1L4
Substrates
Histone H4, 5 µM
SAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L-arginine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- arginine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| SAH | 198 |
| Sinefugin | 173 |
| BIX01294 | >100,000 |
| Chaetocin | >100,000 |
Scientific Information
Q9NR22 (http://www.uniprot.org/uniprot/Q9NR22)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Methyltransferase Assays.
Reference compound IC50 for PRMT8
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