PTPN11/SHP2 (E76K)-FL Phosphatase Assay Service

Full-length PTPN11/SHP2 (E76K) Phosphatase Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.

Target Overview
CAT#

PTPN11/SHP2 (E76K)-FL

Enzyme

Human Full-length PTPN11/SHP2 (E76K)

Synonyms

Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C, SH-PTP2, SH-PTP3, PTP2C, SHPTP2

Substrates

Fluorogenic substrate DiFMUP, 100 μM
With/without activating peptide [H2N-LN(pY)IDLDLV(dPEG8)LST(pY)ASINFQK-amide]

Assay Properties
Reaction

Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
SHP099 (without peptide) 2,600
SHP099 (with peptide) >100,000
Scientific Information

Q06124 (http://www.uniprot.org/uniprot/Q06124)

Screening Location

Malvern, USA

Reference Compound IC50 for PTPN11/SHP2 (E76K)-FL

Reference Compound IC50 for PTPN11/SHP2 (E76K)-FL