PTPN11/SHP2-FL Phosphatase Assay Service

PTPN11/SHP2-FL Phosphatase Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.

CAT#

PTPN11/SHP2-FL

Enzyme

Human Full-length PTPN11/SHP2

Synonyms

Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C, SH-PTP2, SH-PTP3, PTP2C, SHPTP2

Substrates

Fluorogenic substrate DiFMUP, 100 μM
Activating peptide [H2N-LN(pY)IDLDLV(dPEG8)LST(pY)ASINFQK-amide]

Reaction

Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
PHPS1 450
PTP1B Inhibitor 44,300
SHP099 150
Scientific Information

Q06124 (http://www.uniprot.org/uniprot/Q06124)

Screening Location

Malvern, USA

Reference Compound IC50 for PTPN11/SHP2-FL

Reference Compound IC50 for PTPN11/SHP2-FL