PTPN11/SHP2 Catalytic Domain Phosphatase Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
PTPN11/SHP2-CD
Enzyme
Catalytic domain of human PTPN11/SHP2 (aa246-593)
Synonyms
Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C, SH-PTP2, SH-PTP3, PTP2C, SHPTP2
Substrates
Fluorogenic substrate DiFMUP, 10 μM
Assay Properties
Reaction
Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| NSC-87877 | 1,500 |
| PHPS1 | 370 |
| PTP1B Inhibitor | 6,500 |
Scientific Information
Q06124 (http://www.uniprot.org/uniprot/Q06124)
Screening Location
Malvern, USA
Further information
More information can be found on our website Phosphatase Assay Services.
Reference Compound IC50 for PTPN11/SHP2
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