PTPN6/SHP1 Phosphatase Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
PTPN6/SHP1
Enzyme
Human PTPN6/SHP1
Synonyms
Tyrosine-protein phosphatase non-receptor type 6, Hematopoietic cell protein-tyrosine phosphatase Protein-tyrosine phosphatase 1C, Protein-tyrosine phosphatase SHP-1, HCP, PTP1C
Substrates
Fluorogenic substrate DiFMUP, 10 μM
Assay Properties
Reaction
Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| NSC-87877 | 470 |
| PHPS1 | 380 |
| PTP1B Inhibitor | 3,800 |
Scientific Information
P29350 (http://www.uniprot.org/uniprot/P29350)
Screening Location
Malvern, USA
Reference Compound IC50 for PTPN6/SHP1
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