PTPRF/LAR Phosphatase Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
PTPRF/LAR-CD
Enzyme
Human PTPRF (a.a.1340-1630)
Synonyms
LAR
Scientific Information
P10586 (https://www.uniprot.org/uniprotkb/P10586)
Assay Properties
Substrates
Fluorogenic substrate DiFMUP, 10 μM
Reaction
Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate
Readout
Enzymatic activity assay using DiF4MU fluorescence readout
Reference Compound IC50s
| Compounds | IC50 (μM) |
|---|---|
| PTP1B Inhibitor | 2.6 |
| RWJ-60475 | >100 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Phosphatase Assay Services for Drug Discovery
Reference Compound IC50 for PTPRF/LAR
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