RAF1 (R391W) Kinase Assay Service

Activity-based RAF1 (R391W) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#

RAF1 (R391W)

Enzyme

Human RAF1 (R391W)

HGNC

RAF1 (R391W)

Synonym

RAF proto-oncogene serine/threonine-protein kinase, Raf-1, C-RAF, cRaf, RAF, c-RAF

Substrates

MEK1 (K97R), 5 µM
ATP 10 µM

Assay Properties
Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine >100,000
Ro 31-8220 >100,000
GW 5074 5.4
H-89 >100,000
Rottlerin >100,000
Scientific Information

P04049 (http://www.uniprot.org/uniprot/P04049)

Screening Location

Malvern, USA

Reference compound IC50 for RAF1 (R391W)

Reference compound IC50 for RAF1 (R391W)