RAF1 YDYD Kinase Activity Assay Service

Activity-based RAF1 YDYD kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview

RBE Target ID

RAF1 YDYD

Enzyme

Human RAF1 YDYD, wt

HGNC

RAF1 (Y340/Y341D)

Manning ID

RAF1

Synonym

cRaf, RAF1, c-RAF, RAF1 Y340D/Y341D (untagged), RAF1 YDYD

UniProt

P04049 (RAF1_HUMAN)

Kinase Type

Protein kinase

Kinase Group

TKL

Assay Properties

Assay Format

33PanQinaseTM

Substrates

MEK1-K97M, ATP

Reaction

MEK1-K97M + 33P-ATP = 33P-MEK1-K97M + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Sorafenib 120
Sunitinb 100000

Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability

Screening Opportunities
FreeChoice RAF1 YDYD
345 wt Profiler Yes
345 IC50 wt Profiler Yes
Mutant Profiler Addon No
Lipid Kinase Profiler Addon No
IC50 Profiler No
Recombinant Protein Availability
Active recombinant protein

Yes; ProQinaseTM – RAF1 YDYD
(Activity verified by 33PanQinaseTM-Assay)

Product Number

0792-0000-2

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